The immortal chondrocytes were genetically modified by the introduction of ectopic transcription factor, paired box gene 2 (Pax2) driven by the collagen type II alpha 1 promoter. Our objectives were to first investigate the effect of transforming growth factor-β1 (TGF-β1) on the cell proliferation and the organization of immortal chondrocytes. The immortal chondrocytes from human cartilage were used in the present study to attempt to produce stable, elastic, and hyaline-like hydrogels that possess unique properties for use in treating cartilage defects or injuries. Immortal cells are the permanent cells that remain and proliferate despite multiple cell division cycles.

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